Table 2.
Binding Parameters Determined by BLI Compared to Reported Values
| ligand | KD (mM) | ΔG (kcal/mol) | reference ΔG40,60,a | reference ΔG40,60,b |
|---|---|---|---|---|
| 1 | 0.32 (0.02)c | –4.83 (0.04) | –4.9 (0.2) | –4.8 (<0.1) |
| 2 | 0.21 (0.01) | –5.08 (0.02) | ||
| 3 | 0.08 (0.01) | –5.66 (0.04) | –5.5 (0.1) | |
| 5 | 0.22 (0.01) | –5.04 (0.06) | –4.8 (<0.1) | |
| 6 | 0.032 (0.01) | –6.21 (0.14) | ||
| D-Gal | –4.0 (<0.1) |
a
Experiments performed by isothermal titration calorimetry (ITC) at 27 °C.
b
Experiments performed by ITC at 25 °C.
c
Standard deviations shown in parentheses.