Figure 1.

The effects of respiratory complex inhibitors on virus productive infection in MDBK cells. MDBK cells were infected with BoHV-1 (MOI = 1) for 24 h. Throughout infection, the cells were treated with TTFA (a), an inhibitor for mitochondrial RC complex II, NaN₃ (b), an inhibitor for mitochondrial RC complex IV, and oligomycin A (c), an inhibitor for ATP synthase, at indicated concentrations, respectively. After infection, the cell cultures were subjected to frozen-thawing twice, and the viral titer was determined in MDBK cells with the results expressed as TCID₅₀/mL. (d) The cytotoxicity of TTFA (200 μM), NaN₃ (1 mM), and oligomycin A (200 μM) in MDBK cells for 24 h was analyzed by the Trypan-blue exclusion test as described elsewhere [47]. Data represent means of three independent experiments. Significance was assessed with the Student t-test. ^∗p < 0.05.