Depletion of miR-222-3p affects the migration and anti-apoptosis abilities of LoVo/ADR cells. (A, B) Wound healing and Transwell migration assays were performed for LoVo/S cells, LoVo/ADR cells, and LoVo/ADR + miR-222-3p inhibitors cells after treatment with DOX, Scar bar, 50 um. (C) A comparison of the average number of cells penetrating the artificial basement membrane in the migration assays described in B is shown. (D) Annexin V-FITC/PI-positive cells as determined by FlowJo software. (E, F) The total and early apoptotic rates of LoVo/S cells, LoVo/ADR cells, and LoVo/ADR + miR-222-3p inhibitors cells. ** P<0.01, *** P<0.001. DOX – doxorubicin.