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. 2019 Mar 13;116(13):6162–6171. doi: 10.1073/pnas.1814139116

Fig. 5.

Fig. 5.

MiR-122 specifically binds to mouse macrophage endosomal TLR7. (A–C) Macrophage activation by internalized synthetic miR-122 detected by microarray gene-expression profiling (A and B) and qRT-PCR (C). (D, Upper) Comparison of GU enrichment in miR-122 with other TLR7/8-binding miRNAs; (Lower) Detection of miR-122 and miR-29a but not miR-16 in anti-TLR7 antibody-immunoprecipitated complex by qRT-PCR. (E) Colocalization of miR-122-Cy5 and TLR7 in endosomal structures of mouse macrophages. (F, Upper) schematic of NF-κB luciferase reporter assay of miR-122 binding human TLR8 in HEK293T cells. (Lower) Enhancement of NF-κB activity in HEK293T cells by miR-122 and miR-29a via specific binding to human TLR8. Data are presented as mean ± SEM (n = 3). **P < 0.01, ***P < 0.001. Student’s two-tailed, unpaired t test (C) or one-way ANOVA followed by Bonferroni’s multiple comparisons test (D and F).