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Membrane association of GRA proteins correlates with WNG1 kinase activity. (A) Representative Western blot of samples in which host and PV membranes were ultracentrifuged and the soluble (S) and pellet (P) fractions were separated by SDS/PAGE and probed with the indicated antisera. Phosphorylated bands are indicated with arrowheads. (B) Quantification of n = 6–7 biological replicates as in A. Significance was calculated by ANOVA with Tukey’s test in Prism; ***P < 0.001.
