Fig. 1.
P. aeruginosa perturbs mitochondrial function and represses the UPRmt. (A) Images of TMRE-stained wild-type worms following 15 h of exposure to E. coli, P. aeruginosa, or P. aeruginosa-∆gacA. (Scale bar, 0.1 mm.) (B) Oxygen consumption rates (OCRs) of wild-type worms following 12 h of exposure to E. coli, P. aeruginosa, or P. aeruginosa-∆gacA. n = 10; error bars indicate mean ± SD; ****P < 0.03 (Student’s t test). (C) hsp-6pr::gfp worms on E. coli, P. aeruginosa, or P. aeruginosa-∆gacA exposed to a control or 30 μg/mL ethidium bromide. Images were obtained 27 h after exposure. (Scale bar, 0.1 mm.) (D) clk-1(qm30);hsp-60pr::gfp worms exposed to E. coli, P. aeruginosa, or P. aeruginosa-∆gacA for 48 h. (Scale bar, 0.1 mm.) (E) isp-1(qm150);hsp-60pr::gfp worms exposed to E. coli, P. aeruginosa, or P. aeruginosa-∆gacA for 48 h. (Scale bar, 0.1 mm.)