Fig. 2.

The bZIP protein ZIP-3 is a negative regulator of ATFS-1 and the UPR^mt. (A) hsp-6_pr::gfp or zip-3(gk3164);hsp-6_pr::gfp worms on E. coli or pathogenic P. aeruginosa strains PA01, PA14, and CF18²¹. (Scale bar, 0.1 mm.) (B) clk-1(qm30);zip-3(gk3164);hsp-60_pr::gfp and clk-1(qm30);hsp-60_pr::gfp worms on E. coli or P. aeruginosa. (Scale bar, 0.1 mm.) (C) Survival of wild-type and zip-3(gk3164) worms on control or atfs-1(RNAi) exposed to P. aeruginosa. Statistics are in SI Appendix, Table S5. (D) Representative photomicrographs of wild-type, zip-3(gk3164), and zip-3(gk3164);atfs-1(cmh15) worms raised on E. coli and exposed to P. aeruginosa-GFP for 48 h. Images are overlays of differential interference contrast (DIC) and GFP. (Scale bar, 0.1 mm.) (E) Quantification of intestinal colonization of wild-type, zip-3(gk3164), zip-3(gk3164);atfs-1(cmh15), atfs-1(et15), and zip-3_pr::zip-3^Y167A worms. White, gray, and black bars denote no or mild infection, moderate infection, and strong infection, respectively. Thirty worms were analyzed per treatment.