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. 2019 Mar 8;116(13):6146–6151. doi: 10.1073/pnas.1817259116

Fig. 3.

Fig. 3.

ZIP-3 is regulated by proteasomal degradation and represses atfs-1–dependent transcription. (A) Representative photomicrographs of zip-3pr::gfp and zip-3pr::zip-3::gfp worms raised on E. coli. Images are overlays of differential interference contrast (DIC) and GFP. (Scale bar, 0.1 mm.) (B) Representative intestinal images of zip-3pr::zip-3::gfp in worms on control, pbs-1(RNAi), or wwp-1(RNAi). (Scale bar, 0.02 mm.) (C) ZIP-3 protein. (D) clk-1(qm30);hsp-60pr::gfp worms on control or wwp-1(RNAi) and clk-1(qm30);zip-3Y167A;hsp-60pr::gfp on control or zip-3(RNAi). (Scale bar, 0.1 mm.) Quantification is given in SI Appendix, Fig. S3C. (E) atfs-1(et15);hsp-60pr::gfp or atfs-1(et15);zip-3Y167A;hsp-60pr::gfp on control or zip-3(RNAi). (Scale bar, 0.1 mm.) Quantification is given in SI Appendix, Fig. S3D. (F–K) tsfm-1, dnj-10, lys-3, abf-2, ftn-1, and cpt-4 transcripts as determined by qRT-PCR in wild-type or zip-3(gk3164) worms on P. aeruginosa (n = 3, ±SD); *P < 0.05 (Student’s t test). RFU, relative fluorescence unit.