Fig. 2.

Deletion of FAZ5 changes the morphology and swimming of Leishmania cells. (A) Histogram of the pseudodiameter (a measure of cell volume) of the parental, FAZ5 null mutant, and FAZ5 add-back cells measured using a CASY cell counter. (B) Correlation of the cell body length and width of the parental, FAZ5 null mutant, and FAZ5 add-back cells. Fifty 1K1N cells were measured for each cell type. (C) Histogram of flagellum lengths of 1K1N cells for parental (n = 95), FAZ5 null mutant (n = 115), and FAZ5 add-back (n = 72) cells. (D) Swimming tracks from video microscopy of parental, FAZ5 null mutant, and FAZ5 add-back cells (Upper), with histograms of the mean speed and directional persistence for each cell line shown (Lower). (Scale bars, 50 μm.) (Also refer to SI Appendix, Figs. S2 and S3.)