Fig. 3.

FAZ5 deletion reduces endocytosis rate. (A and B) TL and FM4-64 uptake assay with parental and FAZ5 null mutant promastigotes. Promastigotes were incubated with TL and FM4-64 over a 150-min time course and 20-min time course, respectively. Three major categories of TL and FM4-64 localization in the endocytic system were observed: FP; FP and endosome; and FP, endosome, and lysosome. The flagellum membrane protein SMP1 is tagged with EGFP (green). (Scale bars, 5 μm.) (C) Proportion of each category observed at each time point for both parental and FAZ5 null mutant cells for TL and FM4-64 uptake assays. Numbers counted for each time point are indicated above the columns. The uptake assays were done independently three times, and results from a representative experiment are shown. The P value was calculated using a χ² test. (Also refer to SI Appendix, Figs. S4 and S5.)