Fig. 4.

FAZ5 deletion severely affects the ability to proliferate and develop in the sand fly. (A) Analysis of sand fly infections using parental, FAZ5 null mutant, and FAZ5 add-back cells. At 1–2 d after a blood meal and 6–8 d after a blood meal, sand flies were dissected (numbers indicated above the columns) and the parasite load was measured as heavy (1,000+ parasites), moderate (100–1,000 parasites), or weak (1–100 parasites). (B) Location of Leishmania parasites within infected sand flies at 1–2 d and 6–8 d after a blood meal. Stacked columns indicate the percentage of infected sand flies, with parasites in various locations within the sand fly. The FAZ5 null mutant was unable to migrate to the stomodeal valve. The percentage of infected flies for each cell line is indicated above each column.