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. 2019 Mar 8;116(13):6152–6161. doi: 10.1073/pnas.1817924116

Fig. 1.

Fig. 1.

Single-headed KIF1A motors can cooperatively transport membrane-bound cargo in cells. (A) Single-molecule motility assays were carried out using TIRF microscopy to observe 3xmCit-tagged versions of the kinesin-3 motor KIF1A. Representative kymographs are shown for dimeric (Left) and monomeric (Right) KIF1A motors. Time is on the x axis and distance is on the y axis. [Scale bars, 5 s (x axis) and 5 μm (y axis).] (B) Schematic of the inducible peroxisome dispersion assay. A motor-mNG-FRB construct was coexpressed in COS-7 cells with PEX3-mRFP-FKBP. Motors were recruited to peroxisomes via rapamycin (RAP) addition and cells were fixed after 0, 10, or 30 min. Representative images at each time point after RAP addition are shown for (C) dimeric KIF1A and (D) monomeric KIF1A motors. (Scale bars, 10 μm.)