Skip to main content
. 2019 Mar 8;116(13):6152–6161. doi: 10.1073/pnas.1817924116

Fig. 2.

Fig. 2.

Single-headed kinesins can cooperate for peroxisome transport in cells. (A) Single-molecule motility assays of 3xmCit-tagged monomeric versions of motors from the kinesin-1, -2, and -3 families. Representative kymographs are shown. Time is on the x axis; distance is on the y axis. [Scale bars, 5 s (x axis) and 5 μm (y axis).] (B and C) Peroxisome dispersion assay. Representative images of COS-7 cells coexpressing PEX3-mRFP-FKBP and motor-mNG-FRB constructs of (B) dimeric or (C) monomeric versions of the indicated kinesin-1, kinesin-2, or kinesin-3 motors. Shown is the peroxisome distribution in the absence of RAP (Left) or 30 min after RAP addition (Right). See SI Appendix, Figs. S4–S6 for individual fluorescence channels. (Scale bars, 10 μm.)