Fig. 6.

Increasing the motor-to-cargo distance reduces transport by KIF3B monomers in a length-dependent manner. (A) Schematic of constructs. SAH sequences of known length (5, 10, 20, or 30 nm) were fused in-frame after the neck linker of the monomeric KIF3B(1-350) construct. (B) KIF3B motors with extended motor-to-cargo linkers were tested in the peroxisome (Left) and Golgi (Right) dispersion assays. The distribution of each organelle in the absence of rapamycin (−RAP) is shown in the bottom bar of each graph. The numbers in parentheses to the right of each bar indicate the number of cells counted across three independent experiments.