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. 2019 Mar 8;116(13):6152–6161. doi: 10.1073/pnas.1817924116

Fig. 8.

Fig. 8.

Monomeric but not dimeric motors are sensitive to the length and flexibility of the motor-to-cargo linker. A 30nmSAH or 5nmSAH–hinge–20nmSAH motor-to-cargo linker was fused in-frame after the neck linker of (A) the monomeric KIF3B(1-350) construct or (B) the dimeric KIF5BC(1-559) construct and the motors were tested in the peroxisome (Left) and Golgi (Right) dispersion assays. The distribution of each organelle after 30 min of rapamycin (+RAP) treatment is shown in the bar graphs. The numbers in parentheses to the right of each bar indicate the number of cells counted across three independent experiments.