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. Author manuscript; available in PMC: 2019 Jun 5.
Published in final edited form as: Anal Chem. 2018 May 24;90(11):6843–6850. doi: 10.1021/acs.analchem.8b01005

Figure 3.

Figure 3.

(A) Comparison of target binding affinity between PFDM and free DNA. FAM fluorescence variation between PFDM and DNA with addition of cDNA at different concentrations. The concentration of PF–DNA–FAM and DNA–FAM was fixed at 1 μM, and the concentration of cDNA ranged from 5 nM to 1 μM. (B) Melting transition of PFDM/cDNA complex and DNA/cDNA duplex. The concentrations of PF–DNA–FAM, DNA–FAM, and cDNA were fixed at 500 nM. (C) The kinetic fluorescence assays of PFDM/cDNA complex and DNA/cDNA duplex with addition of DNAase I (5 units/mL).