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. Author manuscript; available in PMC: 2019 Aug 8.
Published in final edited form as: J Am Chem Soc. 2018 Jul 25;140(31):9793–9796. doi: 10.1021/jacs.8b04319

Figure 2.

Figure 2.

Self-assembly and dynamic logic operation of 3D DNA- logic gate nanomachine in buffer solution. (a) Dynamic operation of the DNA-logic gate nanomachine. (b) Native-PAGE results confirmed the stepwise assembly of the DNA-logic gate TP. Lane 1: DNA TP scaffold. Lane 2: F/S/R-TP. Lane 3: sgc8c/cS-TP. Lane 4: sgc4f/cF-TP. Lane 5: sgc8c/cS-sgc4f/cF-TP. Lane 6: F/S/R-sgc8c/ cS-sgc4f/cF-TP (DNA-logic gate TP). (c) Determination of the size of 500 nM TP scaffold (red) and DNA-logic gate TP (blue) by DLS. (e) Native-PAGE analysis of the dynamic behavior of the DNA-logic gate TP. Lane 1: DNA-logic gate TP. Lane 2−3: previous sample adding either cS or cF, respectively. Lane 4: DNA-logic gate TP, adding both cS and cF. Lane L: DNA ladder. (f) Ratio optimization of cS and cF. Fluorescence intensity was recorded for FAM-R and BHQ 1-S loaded DNA TP. (g) Fluorescence change of DNA-logic gate TP in response to DNA strand displacement.