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. Author manuscript; available in PMC: 2019 Aug 8.
Published in final edited form as: J Am Chem Soc. 2018 Jul 25;140(31):9793–9796. doi: 10.1021/jacs.8b04319

Figure 4.

Figure 4.

3D DNA-logic gate nanomachine for cell-surface recognition and computing. (a) Flow cytometry reporting signals of different DNA TP-based nanostructures for both CEM and Ramos cells during incubation times of 1 h, 2 and 4 h; A: sgc8c-F/S/R-TP, B: sgc4f-F/S/R-TP, C: sgc8c-sgc4f-F/S/R-TP, D: sgc8c/cS-F/S/R-TP, E: sgc4f/cF-F/S/R-TP, F: sgc8c/cS-sgc4f/cF-F/S/R-TP (DNA-logic gate TP). Only the intact logic gate TP can accomplish the steps of bispecific biomarker recognition, DNA strand displacement reaction- based computing and effective fluorescent reporting. (b) Confocal laser scanning microscopy images of CEM and Ramos cells interacting with the 3D DNA-logic gate nanomachine for 1 h, 2 and 4 h. Scale bar = 10 μm. (c) Truth table of DNA logic-gate nanomachine.