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. Author manuscript; available in PMC: 2019 Oct 31.
Published in final edited form as: J Am Chem Soc. 2018 Oct 16;140(43):14314–14323. doi: 10.1021/jacs.8b08645

Figure 2.

Figure 2.

Primary binding analysis between aptamer candidates and their putative peptide targets. (A-E) Representative predicted secondary structures of aptamer IT1 and its subsequent truncated forms, IT1a, IT1b, IT1c, and IT1d. (F and M–P) Full-length sequences IT1, IT4, IT5, IT6, and IT9 are aptamers that specifically bind to T231 peptide. (F–J) The binding abilities of truncated aptamers IT1a, IT1b, and IT1c to T231 epitope are not seriously compromised when compared to the full-length aptamer IT1. However, binding strength is lost when further truncating the stem of IT1c into IT1d. (K) Sequence IT2 demonstrates a unique binding profile on three of the peptides (T231, T231P, and S202). (L) Sequence IT3 bypasses the phosphorylated site and recognizes both T231 and T231P.