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Aptamer-antibody sandwich ELISA for detection of tau. Both tau and phosphorylated tau can be captured by total tau antibody. However, aptamer IT4 only detects the presence of nonphosphorylated T231 residue. The scrambled random sequence (RS) and phosphorylated tau protein are included in this test as negative controls. All the sequences are labeled with biotin, which later reacts with streptavidin-labeled polyHRP enzyme and TMB substrate to reveal the colorimetric reaction products.
