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. Author manuscript; available in PMC: 2019 Aug 13.
Published in final edited form as: Nature. 2019 Feb 13;566(7744):383–387. doi: 10.1038/s41586-019-0948-2

Figure 4. Hematopoietic CSF1 deletion or hypocretin supplementation protects against SF-induced hematopoiesis and atherosclerosis.

Figure 4.

a, Schematic of chimeric Ldlr−/− mice with WT or CSF1−/− hematopoietic cells subjected to SF. b, Enumeration of blood Ly-6Chi monocytes in chimeric mice (n=5 LDLr−/−bmWT; n=6 LDLr−/−bmWTSF; n=6 Ldlr−/−bmCsf1−/−; n=5 Ldlr−/−bmCsf1−/−SF ). c, Quantification of bone marrow LSKs and BrdU incorporation in chimeric mice (for LSKs/leg n=5 LDLr−/−bmWT; n=6 LDLr−/−bmWTSF; n=6 Ldlr−/−bmCsf1−/−; n=5 Ldlr−/−bmCsf1−/−SF; for proliferation n=4 LDLr−/−bmWT; n=6 LDLr−/−bmWTSF; n=6 Ldlr−/−bmCsf1−/−; n=5 Ldlr−/−bmCsf1−/−SF). d, BM CSF1 concentration in chimeric mice (n=4 LDLr−/−bmWT; n=4 LDLr−/−bmWTSF; n=5 Ldlr−/−bmCsf1−/−; n=4 Ldlr−/−bmCsf1−/−SF). e, Cross section images of aortic roots stained with oil-red-o and assessment of atherosclerosis in chimeric mice after 16 weeks of high cholesterol diet (n=5 LDLr−/−bmWT; n=6 LDLr−/−bmWTSF; n=6 Ldlr−/−bmCsf1−/−; n=6 Ldlr−/−bmCsf1−/−SF). f, Schematic of mice subjected to SF and receiving hypocretin-1 or saline via osmotic mini-pumps for 8 weeks. g, Enumeration of blood Ly-6Chi monocytes and neutrophils (n=5 Apoe−/−; n=5 Apoe−/−SF; n=7 Apoe−/−+HCRT-1; n=9 Apoe−/−SF+HCRT-1). h, LSKs and BrdU incorporation in the bone marrow of SF mice with HCRT-1 supplementation (n=5 Apoe−/−; n=4 Apoe−/−SF; n=7 Apoe−/−+HCRT-1; n=9 Apoe−/−SF+HCRT-1). i, BM CSF1 levels (n=4 Apoe−/−; n=3 Apoe−/−SF; n=3 Apoe−/−+HCRT-1; n=4 Apoe−/−SF+HCRT-1). j, Cross section images of aortic roots stained with oil-red-o and quantification of atherosclerotic lesion area (n=6 Apoe−/−; n=8 Apoe−/−SF; n=7 Apoe−/−+HCRT-1; n=9 Apoe−/−SF+HCRT-1). k, Model of sleep’s role in regulating hypocretin production, hematopoiesis and atherosclerosis. The illustration was modified from Servier Medical Art (http://smart.servier.com/), licensed under a Creative Common Attribution 3.0 Generic License. Data presented as mean ± s.e.m., *p<0.05, **p<0.01,***p<0.001, One-way ANOVA.