Fig. 1.

Higher glycolysis phenotype in CAFs compared with NFs. a. Heat-Map of the dysregulated genes involved in energy metabolism in 6 paired breast CAFs and NFs detected by Agilent mRNA microarrays. Red or green indicates the up-regulated or down-regulated genes, respectively (Fold changes>1.8; P < .05, CAFs vs NFs). b. The protein levels of some metabolism-related genes were detected with the indicated antibodies. β-Actin is the loading control. c-d. The OCR from OxPhos (c) and ECAR from glycolysis (d) of NFs and CAFs were detected (*, CAFs vs NFs, P < .05, Student's t-test). e. JC-1 staining was used to detect mitochondrial membrane potential of NFs and CAFs, the quantification was detected by flow cytometer. Scale bar, 50 μm. f. Graphs detected by transmission electron microscope show the numbers of mitochondrial and mitochondrial crista in NFs and CAFs. Red arrows indicate the numbers of mitochondrial (left panels, scale bar, 2 μm) or mitochondrial crista (right panels, scale bar, 0.5 μm). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)