FIGURE 2.

Single cell RNA analysis identifies multiple pulmonary cell types. A: four major cell types (left panel) and 18 subtypes (right panel) were identified by RNA analysis using “Drop-seq” of single cells (n = 8,090) from mouse lung at postnatal day 3 (PND3). B: heatmap shows the expression of the predicted cell type signature genes corresponding to cell type. Numerical values 3–18 represent cell clusters defined in A. C: hierarchical clustering reconstructs major lung cell types predicted from the RNA data. Endo, endothelial cells; Mesen, mesenchymal cells; Epi, epithelial cells; Immune, immune cells; FB, fibroblast cells; AT1, alveolar type 1 cells; AT2, alveolar type 2 cells; MyoFB, myofibroblast cells; SMC, smooth muscle cells; MatrixFB, matrix fibroblast cells.