Figure 5.

Small-molecule inhibitors of aerobic glycolysis and the pentose phosphate pathway impair pulmonary embolism and hemostasis. (A) Representative light microscopy images (100X magnification) of hematoxylin & eosin-stained lung sections from mice administered collagen (1 mg/kg) plus epinephrine (10 μg/kg) after pre-treatment with vehicle (control), DCA, DHEA, or DASA as indicated. Black arrows indicate thrombi within the lumen of pulmonary vessels. Yellow arrows indicate extravasated erythrocytes. (B and C) Scatter dot plots showing number of thrombosed pulmonary vessels per low power field and tail-bleeding times, respectively, of mice administered with vehicle, DCA, DHEA or DASA. Data are presented as the mean ± standard error of mean. Each dot represents an independent observation. DASA: diarylsulfonamide; DCA: dichloroacetate; DHEA: dehydroepiandrosterone sulphate.