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. 2019 Mar 5;10(19):1812–1828. doi: 10.18632/oncotarget.26737

Figure 2. Vaccine-activated CD4+ T cells secrete various Th1 and Th2 type cytokines.

Figure 2

Purified CD4+ T cells from HLA-DR1 positive donors were primed with Mel202/DR1/CD80 and boosted with Mel202/DR1/CD80 (CD4 + vac) or parental Mel202 (CD4 + wt) cells. Th1 (IFNγ, TNFα and TNFβ) and Th2 type (IL-5, IL-6, IL-8, IL-10) cytokine release in the supernatant was measured by a flowcytometric multiplex bead array. Purity of CD4+ T cells was >90% throughout the course of the experiments. Data are pooled from three independent experiments with purified CD4+ T cells for each donor 1, 2 or 3.