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. 2019 Mar 5;10(19):1812–1828. doi: 10.18632/oncotarget.26737

Figure 4. Vaccine-activated CD4+ T cells display a diverse polyclonal TCR-Vβ repertoire.

Figure 4

Purified CD4+ T cells were primed and repetitively boosted (7x) with Mel202/DR1/CD80 vaccine cells. CD3+/CD4+ T cells were gated and analyzed by flow cytometry for TCR-Vβ usage on day 0 and after boosts 1, 3, 5, and 7. Each colored bar denotes the different time points of analysis. Purified CD4+ T cells from 3 individual donors were used. Purity of CD4+ T cells was >90% throughout the course of the experiments. Data are representative of two independent experiments for each donor 1, 2, and 3.