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. 2019 Mar 5;10(19):1812–1828. doi: 10.18632/oncotarget.26737

Figure 5. Vaccine-activated CD4+ T cells maintain expression of proliferation and activation markers.

Figure 5

Purified CD4+ T cells were primed and repetitively boosted (7x) with Mel202/DR1/CD80 vaccine cells. CD3+/CD4+ T cells were gated and analyzed by flow cytometry for expression of (A) Ki67 and (B) HLA-DR on day 0, after prime and boosts 1-7. Purity of CD4+ T cells was >90% throughout the course of the experiments. Data are representative of two independent experiments with purified CD4+ T cells for each donor 1, 2, and 3.