Table 4. Primers used for sequencing selected exons of the miRNA processing genes.
| Gene | Transcript ID | Exon | Functional domain | Primers |
|---|---|---|---|---|
| DICER1 | ENST00000343455.7 | 24 | RNase IIIb | F: AAGCTTACGGTTCCACTTCG |
| R: ACCACTATGCCGTCAGAACT | ||||
| 25 | F1: AGAATAATAAATGGGGTGGGGAT | |||
| R1: GTACACCTGCCAGACTGTCT | ||||
| F2: AACTACATCTGTGGACTGCC | ||||
| R2: TCAAGCAATTCTCGCACAGG | ||||
| DROSHA | ENST00000511367.6 | 23 | RNase IIIa | F: TGTGTTCTTTTCTTGCGGGG |
| R: ACACGGTGTATCAATGCCTT | ||||
| 24 | F: TCAGAGCCACAGACAGAATGT | |||
| R: ACCGCAGAAGAGCATGTCA | ||||
| 29 | RNase IIIb | F: TCAATCGAGGGGCCTTAGG | ||
| R: TGGAGGAAGTGATTTAACCAACT | ||||
| 30 | F: TGTTCTCAATGTACCGCCAT | |||
| R: AGGAGGAGGACAAATACGGT | ||||
| DGCR8 | ENST00000351989.7 | 7 | heme- and pri-miRNA binding | F: GTGGCACTGCTTCACACTTG |
| R: GCCCTGACCAAAGTTACACC | ||||
| TARBP2 | ENST00000266987.6 | 7 | DICER1- and pre-miRNA binding | F: GGTCTGTGGGGAATCATAACC |
| R: CAGAAGCAGACCTAGGGCC | ||||
| 8 | F: TCGCTTCATCTTTCTCACTGT | |||
| R: CCCTTCTACTTGCTCCGGTC |