Figure 1. Ibrutinib-induced inhibition of inside-out and outside-in signaling of LFA-1 in monocytes.

Shown are control cells (Ctrl) versus Ibrutinib-treated cells (Ibrutinib). (A) Number of monocytes adherent to ICAM-1 upon triggering with buffer (resting) or 25 nM fMLP. (B) Number of monocytes spread on ICAM-1 upon triggering with 1 mM MnCl₂ for 10, 20, 30 minutes. Data presented are mean ± SD and are representative of 8 experiments. ^*p < 0.001 by one-way analysis of variance and Bonferroni post-hoc analysis. (C) Representative image of spread monocytes on ICAM-1 upon triggering with 1 mM MnCl₂ for 10 minutes. Images are captured by contrast phase microscope (10x) and are representative of 3 experiments.