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. 2019 Feb 28;41:556–570. doi: 10.1016/j.ebiom.2019.02.035

Table 1.

Primers for real-time PCR.

Source Name Forward Reverse
Human ADAMTS-5 5′-GAACATCGACCAACTCTACTCCG-3′ 5′-CAATGCCCACCGAACCATCT-3′
MMP-13 5′-ACTGAGAGGCTCCGAGAAATG-3′ 5′-GAACCCCGCATCTTGGCTT-3′
IL-1β 5′-ATGATGGCTTATTACAGTGGCAA-3′ 5′-GTCGGAGATTCGTAGCTGGA-3′
IL-6 5′-ACTCACCTCTTCAGAACGAATTG-3′ 5′-CCATCTTTGGAAGGTTCAGGTTG-3′
iNOS 5′-CAGGGTGTTGCCCAAACTG-3′ 5′-GGCTGCGTTCTTCTTTGCT-3′
Cortistatin 5′-CGGCAGGAATAAGGAAAAGCA-3′ 5′-TGGGAGGTCCACTCAAACCA-3′
Aggrecan 5′-ACTCTGGGTTTTCGTGACTCT-3′ 5′-ACACTCAGCGAGTTGTCATGG-3′
Collagen 2 5′- TGGACGATCAGGCGAAACC-3′ 5′- GCTGCGGATGCTCTCAATC -3′
NF-κB 1 5′-TGGGCACAAGTCGTTTATGA-3′ 5′-CTGGAGCCGGTAGGGAAG-3′
p-IκBα 5′-CATTGGTTCAGAACATGGCCT-3′ 5′-AGCTGTTGTGTGCTGAGACTG-3′
GAPDH 5′-GGAGCGAGATCCCTCCAAAAT-3′ 5′-GGCTGTTGTCATACTTCTCATGG-3′
Mouse ADAMTS-5 5′-CCCAGGATAAAACCAGGCAG-3′ 5′-CGGCCAAGGGTTGTAAATGG-3′
MMP-13 5′-TGTTTGCAGAGCACTACTTGAA-3′ 5′-CAGTCACCTCTAAGCCAAAGAAA-3′
IL-1β 5′-GAAATGCCACCTTTTGACAGTG-3′ 5′-TGGATGCTCTCATCAGGACAG-3′
IL-6 5′-CTGCAAGAGACTTCCATCCAG-3′ 5′-AGTGGTATAGACAGGTCTGTTGG-3′
iNOS 5′-CTCTTCGACGACCCAGAAAAC-3′ 5′-CAAGGCCATGAAGTGAGGCTT-3′
Cortistatin 5′-GAGCGGCCTTCTGACTTTCC-3′ 5′-GGGCTTTTTATCCAGGTGTGG-3′
Aggrecan 5′-CCCAGGATAAAACCAGGCAG-3′ 5′-CGGCCAAGGGTTGTAAATGG-3′
Collagen 2 5′-GGGTCACAGAGGTTACCCAG-3′ 5′-ACCAGGGGAACCACTCTCAC-3′
NF-κB 1 5′-CCTGGAACCACGCCTCTA-3′ 5′-GGCTCATATGGTTTCCCATTTA-3′
p-IκBα 5′-ATGCAGAGTACCACTAACTACCT-3′ 5′-CCTCCCCGGATTTCTTGTTTC-3′
GAPDH 5′-AGCAGTCCCGTACACTGGCAAAC-3′ 5′-TCTGTGGTGATGTAAATGTCCTCT-3′