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(A) Structures of RFA, RCA and RIBFA. (B) Inhibitor screens using RFA. (C) Structures of Cl/F-amidine-based azide and Yne; fluorescein-based reporter tags (azide and Yne); biotin-azide, in which biotin and the azide functionalities were installed between a TEV protease site. (D) Structures and potencies of BB-F-Yne and BB-Cl-Yne for PAD1–4. (E) Labeling of a PAD by BB-X-Yne (X = F, Cl) in mammalian cells.
