Table 2. Lactate production of cell lines cultured in the media without or with 0.05mg/ml gentamicin addition.
L-lactate concentration (μM) was measured by Cayman glycolysis cell-based assay kit.
| Cell line | Amount of cells | Media without gentamicin | Media with 0.05mg/ml gentamicin | p2 |
|---|---|---|---|---|
| MCF-12A | 10,000 | 2.29±0.41(3)1 | 3.35±0.45(3) | <0.05 |
| MCF-7 | 10,000 | 3.46±0.45(3) | 4.25±0.40(3) | >0.05 |
| MDA-MB-231 | 10,000 | 3.39±0.38(3) | 4.28±0.54(3) | >0.05 |
1 Mean ± SD (N)
2 Unpaired t-test, less than 0.05 as significant; NS: not significant