Table 3. DNA oxidative damage of cell lines cultured in the media without or with 0.05mg/ml gentamicin addition.
8-OHdG concentration (pg/mL) in the media was measured by DNA Damage Competitive assay kit.
| Cell line | Amount of cells | Media without gentamicin | Media with 0.05mg/ml gentamicin | p2 |
|---|---|---|---|---|
| MCF-12A | 10,000 | 248±65(3)1 | 375±41(3) | <0.05 |
| MCF-7 | 10,000 | 353±49(3) | 445±0.46(3) | >0.05 |
| MDA-MB-231 | 10,000 | 351±48(3) | 449±0.45(3) | >0.05 |
1 Mean ± SD (N)
2 Unpaired t-test, less than 0.05 as significant; NS: not significant