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. 2019 Apr 1;14(4):e0211170. doi: 10.1371/journal.pone.0211170

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© 2019 Mastromoro et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — A.B: Whole mount fluorescent photographs of the outflow region of a E18.5 Tbx1^cre/+; Rosa^mT/mG fetus. A: external appearance. B: internal optical plane. Note the heavy contribution of GFP+ cells to the pulmonary myocardium, pulmonary trunk (PT) and pulmonary valves, but superficial contribution (endothelial and adventitial) to other great vessels, including the ductus arteriosus (DA). which appears to have a more dense endothelial contribution. aAo: ascending aorta. C: immunofluorescence of a transverse section of a E18.5 Tbx1^cre/+; Rosa^mT/mG. Anti GFP staining is shown in green, anti PECAM1 staining (endothelial-specific) is shown in red. DAPI staining (cell nuclei) is shown in blu. C'-C'': green and red channels are shown separately. aAo: ascending aorta; PT: pulmonary trunk and pulmonary leaflets; LPA, RPA: left and right pulmonary arteries. Scale bar is 50 micrometers in A and B, 100 micrometers in C-C".