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. 2019 Mar 20;13(3):e0007259. doi: 10.1371/journal.pntd.0007259

Fig 6. Comparison of relationship between staining efficacy and parasite burden in samples acquired using BD LSRFortessa, BD LSRFortessa X-20 or BD FACSCanto II flow cytometer.

Fig 6

Samples acquired with BD LSRFortessa (A), BD LSRFortessa X-20 (B) or BD FACSCanto II (C) flow cytometer were divided by percentage of Alexa 488+ events among events in Oocyst gate (staining efficacy). Parasite burden of samples categorized previously by staining efficacy and acquired with BD LSRFortessa (D), BD LSRFortessa X-20 (E) or BD FACSCanto II (F) flow cytometer. Staining efficacy (A to C) is inversely proportional to parasite burden (D to F) in same samples. ** (p < 0.01); *** (p < 0.001); **** (p < 0.0001).