Fig 3. Altered testes morphology and impaired spermatogenesis as well as ovarian morphology and follicle maturation at 6–7 months of age.

(A–B) Histological images of representative IR^loxp and IRKO^GFAP testes. (C–F) Analysis of number of spermatogonium, spermatocyte, spermatid, and spermatozoa of IR^loxp (n = 5) and IRKO^GFAP (n = 4). IR^loxp (black bar) and IRKO^GFAP (white bar). Values are expressed as means ± SEM. *P < 0.05 IRKO^GFAP versus the IR^loxp group. Histological images of IR^loxp (n = 4) and IRKO^GFAP (n = 3) female mice IR^loxp (black bar) and IRKO^GFAP (white bar). (I–M) Ovarian follicle maturation analysis of different follicle stages (primordial, primary, secondary, and Graafian) and corpora lutea in IR^loxp (n = 4) and IRKO^GFAP (n = 3) mouse ovaries. Values are expressed as means ± SEM. *P < 0.05 IRKO^GFAP versus the IR^loxp group. The underlying data can be found in S1 Data. IR, insulin receptor; IRKO^GFAP, astrocyte-specific insulin receptor deletion.