Table 1.
Plate thickness
| Plate | Microscopea | Phase plate | Binning | Thicknessb (nm) |
|---|---|---|---|---|
| Monolayer | Polara | − | No | 7.2 ± 1.4 (n = 87) |
| Polara | − | 4× | 8.8 ± 2.0 (n = 85) | |
| Krios | + | 4× | 7.2 ± 1.0 (n = 366) | |
| Krios | − | 4× | 7.3 ± 0.9 (n = 39) | |
| Two layers in contact | Polara | − | No | 13.0 ± 2.0 (n = 26) |
| Krios | + | 4× | 12.4 ± 1.7 (n = 126) | |
| Krios | − | 4× | 13.0 ± 1.1 (n = 13) |
a
Tecnai Polara (27,500×); Titan Krios (33,000×).
b
Thickness measurements account for CTF fringes as described in Fig EV2. Values shown are means ± SD of the indicated number (n) of independent measurements.