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. 2019 Feb 25;38(7):e99895. doi: 10.15252/embj.201899895

Figure EV3. The effect of manipulating histidine metabolic pathway intermediates on control and nerfin‐1 clones (related to Figs 3 and 4).

Figure EV3

  • A
    500 mg/ml of the histamine receptor inhibitor cimetidine (n = 39, 23) did not significantly alter nerfin‐1 clonal growth in the adult CNS.
  • B
    Knockdown of histamine inhibitor HisCl1 (HisCl1Ri), knockdown verified in Oh et al, 2013, did not significantly alter nerfin‐1 clonal growth in the larval CNS (n = 37, 42).
  • C
    Hdc knockdown did not significantly alter the growth of wildtype larval CNS clones (n = 42, 36).
  • D
    Histamine supplementation did not significantly increase nerfin‐1 clone size, but significantly rescued nerfin‐1;HdcRi clonal growth in the larval CNS (n = 15, 10, 12, 9).
  • E
    Hdc knockdown did not significantly alter the amount of cell death in nerfin‐1 clones in the larvae (n = 43, 35).
  • F
    The speed of the cell cycle in fully dedifferentiated NBs > 8 μm (n = 11, 9) and dedifferentiating neurons < 8 μm (n = 11, 9) was not significantly altered by Hdc inhibition in nerfin‐1 larval clones.
  • G–I
    Representative pictures showing that Hdc inhibition significantly reduced the % of Myc+ NBs (red) and Myc+ Elav+ (blue) neurons in nerfin‐1 larval clones (G–H’’), quantified in (I) (n = 14, 14, 13, 16). Scale bar = 50 μm.
Data information: In all graphs, the key indicates that green bars represent a significant increase (P < 0.05), red bars a significant decrease (P < 0.05), and grey bars no significant change (P > 0.05) in t‐tests with the relevant paired controls (black bar). In all graphs, error bars represent 1 standard error of the mean (SEM). FC, fold change.