Figure EV4. Hdc knockdown reduces N^ACT larval type II CNS clones, but not the growth of N^ACT and scrib ¹ ;Ras ^V12 larval eye imaginal disc clones (related to Figs 1 and 4).

• A–C
  Representative pictures showing that larval type II N^ACT MARCM clonal growth was significantly reduced by Hdc (A–B’’) and Myc knockdown, quantified in (C) (n = 11, 7, 7). Scale bar = 50 μm.
• D–G
  Representative pictures showing that Myc (F) and Hdc (E) inhibition was sufficient to significantly reduce N ^ACT clonal growth in the larval eye imaginal epithelia, quantified in (G) (n = 75, 80, 75). Scale bar = 100 μm.
• H–J
  Hdc inhibition did not significantly alter the growth of scrib ¹ ;Ras ^V12 larval eye imaginal epithelia clones, quantified in (J) (n = 57, 33). Scale bar = 100 μm.

Data information: In all graphs, the key indicates that green bars represent a significant increase (P < 0.05), red bars a significant decrease (P < 0.05), and grey bars no significant change (P > 0.05) in t‐tests with the relevant paired controls (black bar). In all graphs, error bars represent 1 standard error of the mean (SEM). FC, fold change.