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. 2019 Mar 8;38(7):e100353. doi: 10.15252/embj.2018100353

Figure 6. LRX and FERONIA jointly sense extracellular signals.

Figure 6

  1. Rosette phenotype of 3‐week‐old Col‐0, fer‐4, lrx3/4/5 and fer‐4/lrx3/4/5 (upper panel). Vacuolar morphology (MDY‐64‐stained) of late meristematic atrichoblast cells of Col‐0, fer‐4, lrx3/4/5 and fer‐4/lrx3/4/5 (lower panel). Scale bars: 1 cm (upper row) and 5 μm (lower row).
  2. LRR4‐HA and RALF1‐FLAG were transiently expressed (as indicated by + and −) in Nicotiana benthamiana. Immunoprecipitation and subsequent detection of the proteins by Western blotting were done as labelled.
  3. Relative root length [% of control] of Col‐0 (n = 11–13), lrx3/4/5 (n = 9–13) and fer‐4 (n = 11–12) after 3 days of RALF1 treatment. Statistical analyses were performed for each concentration using one‐way ANOVA followed by Bonferroni post test (1 and 1.25 μM b: P < 0.001; 1.5 μM b: P < 0.05, c: P < 0.001). Boxplots: Box limits represent 25th percentile and 75th percentile; horizontal line represents median. Whiskers display min. to max. values. Representative experiment is shown.
  4. Nicotiana benthamiana was transiently transformed with LRR4‐HA and NtermFER‐FLAG. Immunoprecipitation and subsequent detection of the proteins by Western blotting were done as labelled.
  5. Yeast was transformed with either empty plasmids (e/e), NtermFER‐FLAG or LRR4‐HA with the empty plasmid, or both NtermFER‐FLAG and LRR4‐HA. Growth of yeast on quadruple drop‐out medium was only observed in cells transformed with NtermFER‐FLAG and LRR4‐HA.

Source data are available online for this figure.