Table 1.
Generation times (Tn), lag phase times, yields of carbon assimilated per cell, measured cell diameters, yields of carbon assimilated per unit cell volume, and yields of carbon assimilated per μJ of energy released in cultures of Acidianus sp. DS80 grown at 80°C in base salts medium (pH 3.0) with CO2 as sole carbon source with the electron donors and acceptors specified. The average and standard deviation of three replicate cultures is presented. In the case of cell diameter determinations, the average and standard deviation of measurements of >10 cells, based on TEM, are presented.
| Redox reaction | Tn (hrs) | Lag phase (hrs) | fmol CO2 /cell | Cell diameter (nm) | fmol CO2 /m3 b | fmol CO2 /μJ |
|---|---|---|---|---|---|---|
| H2(g) + S°(s) → H2S(aq) | 35 ± 17 | 24 | 3.00 ± 0.34 | 1038 ± 68 | 4.41 ± 3.24 | 267.4 ± 36.2 |
| S°(s) + 6Fe3+(aq) + 4H2O(aq) → 6Fe2+(aq) + HSO4−(aq) + 7H+ | 33 ± 19 | 96 | 3.44 ± 1.90 | 1128 ± 38 | 4.39 ± 2.92 | 31.7 ± 1.7 |
| H2(g) + 2Fe3+(aq) → 2Fe2+(aq) + 2H+ | 27 ± 4 | 120 | 1.64 ± 0.23 | 826 ± 55 | 4.85 ± 2.87 | 34.2 ± 5.0 |
| Competition Experiment (H2 / S° / Fe3+)a | 31 ± 2 | 24 | 3.32 ± 0.25 | 1017 ± 29 | 5.87 ± 1.83 | N/D |
Redox reaction is not defined since multiple potential electron donors and acceptors are present in the cultivation medium.
Calculated assuming a spherical (coccoid) morphology (Fig. 1).
Abbreviations: N/D, not determined.