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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Exp Mol Pathol. 2012 Apr 18;93(1):91–98. doi: 10.1016/j.yexmp.2012.04.014

Fig. 1. Effect of CARD-024 on TGFβ stimulated colonic myofibroblasts.

Fig. 1

(A) Representative Western blot of αSMA protein expression in Ccd-18co cells stimulated with increasing doses of TGFβ (0.1 – 2 ng/ml). (B) Representative Western blot illustrating αSMA protein expression in response to treatment with TGFβ and increasing amounts of CARD-024 (10 – 1000 nM) compared to TGFβ treated or untreated cells. In (A) and (B) GAPDH expression is used as a protein loading control. (C) Quantification of αSMA protein expression as normalized to GAPDH in Ccd-18co cells treated with TGFβ and 10–1000 nM CARD-024. Results are from three independent experiments. (* p <0.05, *** p <0.001).