Fig. 1.

Degradation of RNA in response to poly(I:C) in dTHP-1 cells does not require OAS1 or OAS3. (A) Knock-out of OAS1, OAS3, or RNaseL in HT1080 cells that were stimulated with 10 μg/ml poly(I:C) overnight was confirmed by immunoblot. (B) WT and KO HT1080 cells were transfected with 1 μg/ml poly(I:C) for 6 h, and then lysed. RNA integrity was assessed by Bioanalyzer. The positions of 18S and 28S rRNA are indicated. (C) OAS1^−/− and OAS3^−/− dTHP-1 cells were treated with 10 μg/ml poly(I:C) overnight. Cells were lysed, and expression of OAS1 and OAS3 was analyzed by immunoblot. (D) WT and KO dTHP-1 cells were transfected with 1 μg/ml poly(I:C) for 6 h, and RNA integrity was analyzed. *P < 0.05 (two-tailed unpaired Student’s t-test).