Fig. 2.

OAS3 regulates intracellular poly(I:C)-induced expression of chemokines and interferon-stimulated genes in THP-1 cells. (A) Changes in gene expression in WT, OAS1^−/−, and OAS3^−/− dTHP-1 cells transfected with 1 μg/ml poly(I:C) for 6 h. The heat map represents genes that were increased (a, 162 genes) or decreased (b, 82 genes) by more than twice in OAS3^−/− cells compared with WT cells. (B) Heat map depicting the expression of genes related to type I interferon signaling and chemokine activity in WT, OAS1^−/−, and OAS3^−/− dTHP-1 cells stimulated with intracellular poly(I:C). Relative gene expression compared with WT cells is shown for each independent sample. Over-expression is shown in red, under-expression in blue. (C) WT, OAS1^−/−, and OAS3^−/− dTHP-1 cells were transfected with 1 μg/ml poly(I:C) for 6 h, and the mRNA levels of the indicated genes were quantified by qRT-PCR. Data are representative of at least three independent experiments. *P < 0.05 (two-tailed unpaired Student’s t-test).