Figure 8.

Conditional knockout of Fam20c leads to alterations in the distribution pattern of BMP4 and the expression of BMP signaling pathway components in the PG and SLG IHC of BMP4 in the (A) PG and the (B) SLG. In control mice, BMP4 was localized to the cytoplasm of duct cells (black arrows). For images of Fam20c^f/f or Fam20c^f/f; Mmtv-cre mice, the second panel exhibits magnified images of the outlined areas from the corresponding panel. IHC of BMP signaling pathways in the PG and the SLG. (C) IHC of pan-ERK and pan-p38 in the PG. (D) IHC of p-ERK and p-p38 in the PG. (E) IHC of pan-ERK and pan-p38 in the SLG. (F) IHC of p-ERK and p-p38 in the SLG. IHC reaction to pan-ERK and pan-p38 were detected to the cytoplasm of duct cells (black arrows), however the p-Erk and p-p38 were observed in the PG (red arrows) but not in the SLG (red arrows). IHC, immunohistochemistry; BMP, bone morphorgenic protein; p-, phosphorylated; ERK, extracellular signal regulated kinase; PG, parotid gland; SLG, sublingual gland; Fam20C, family with sequence similarity 20-member C.