Table I.
Gene | Forward primers | Reverse primers |
---|---|---|
Floxed allele | a: TCCAGCTTGCTAGGGCTCTGACC | b: CTATGTCCAACGGCCGCAGCTT |
c: GTCCTGAGGGCTGACCCAAGACTA | ||
Mmtv-Cre transgene | AGCGATGGATTTCCGTCTCTGG | AGCTTGCATGATCTCCGGTATTGAA |
Cre-loxP recombination | GTGGTCTCTGCCGCTGATGTACC | TTTGGGAGCCTATGTCCAACGGCC |
Bmp2 | TGACTGGATCGTGGCACCTC C | AGAGTCTGCACTATGGCATGGTTA |
Bmp4 | ACAATGTGACACGGTGGGAAAC | TGTGGGTGATGCTTGGGACTAC |
Bmp7 | ACATCCGGGAGCGATTTGAC | TCCTCAGAAGCCCAGATGGTC |
β-actin | AGAGGGAAATCGTGCGTGAC C | TCGTTGCCAATAGTGATGACC |
BMP, bone morphogenic protein.