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Sirt1 attenuates hypoxia-induced apoptosis in H9C2 cells. (A) Representative western blot images for the expression of apoptosis-related proteins in H9C2 cells subjected to the indicated treatments. (B) Densitometric analysis of the cleaved Caspase3 and Bcl2 levels. (C) Apoptosis was analysed by Annexin V-PE/7-AAD flow cytometry. n=3. *P<0.05 vs. Normoxia + Ad-LacZ group; #P<0.05 vs. Hypoxia + Ad-LacZ group. PE, phycoerythrin; 7-AAD, 7-aminoactinomycin; sh, short hairpin.
