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. 2019 Mar 12;43(5):2133–2143. doi: 10.3892/ijmm.2019.4131

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effects of MRF on H/R-induced apoptosis. (A) Scatter diagram of JC-1 staining, as measured using flow cytometry. (B) Scatter diagram of Annexin V-PI double staining, as measured using flow cytometry. (C) The quantitative analysis of the ratio of red to green fluorescence intensity. (D) The quantitative analysis of apoptosis rate. (E) Caspase-3 activity was measured using a fluorometric assay. The data are presented as the mean ± standard deviation from three independent experiments. ^#P<0.05 vs. the control, ^##P<0.01 vs. the control; ^*P<0.05 vs. H/R-treated cells, ^**P<0.01 vs. H/R-treated cells. MRF, Myrica rubra flavonoids; H/R, hypoxia/reoxygenation; PI, propidium iodide.