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. 2019 Mar 14;43(5):2044–2054. doi: 10.3892/ijmm.2019.4134

Figure 1.

Figure 1

Low expression of Keap1 in RCC is associated with a poor survival rate. (A) In total, 45 cases of patients with RCC were analyzed to determine the mRNA expression of Keap1 in normal and cancer tissues (P=0.001). (B) Kaplan-Meier survival curve was determined to assess the association between the expression of Keap1 and survival rate (P=0.140). A total of five patients with RCC were randomly selected to assess the expression of Keap1 at the (C) protein and (D) mRNA level. GAPDH served as an internal control. Data are presented as the mean ± standard deviation from three independent experiments. **P<0.01 vs. respective normal RCC tissue. (E) Western blotting was used to detect the protein expression in normal human renal tubular epithelial cells (HK-2 cells) and five RCC cell lines (ACHN, 769-P, A-498, 786-0 and Caki-1). (F) Reverse transcription-quantitative polymerase chain reaction was used to detect the mRNA expression in normal human renal tubular epithelial cells and five RCC cell lines. GAPDH served as an internal control. Data are presented as the mean ± standard deviation from three independent experiments. **P<0.01 vs. HK-2 cells. Keap1, Kelch-like ECH-associated protein 1; RCC, renal cell carcinoma.