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. 2019 Mar 14;43(5):2044–2054. doi: 10.3892/ijmm.2019.4134

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Copyright: © Huang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Transfection efficiencies of Keap1 and Nrf2 in ACHN cells. (A) Keap1 was silenced and overexpressed in cells and the mRNA expression of Keap1 was determined by RT-qPCR. (B) Nrf2 was silenced and overexpressed in cells and the mRNA expression of Nrf2 was determined by RT-qPCR. Data are presented as the mean ± standard deviation from three independent experiments. ^**P<0.01 vs. control. Keap1, Kelch-like ECH-associated protein 1; Nrf2, nuclear factor erythroid 2-related factor 2; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; si, small interfering; NC, negative control.